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UID:20251108T083917EST-47838BPhka@132.216.98.100
DTSTAMP:20251108T133917Z
DESCRIPTION:By beating the diffraction limit that restricts traditional lig
 ht microscopy\, single-molecule fluorescence imaging is a precise\, noninv
 asive way to sensitively probe position and dynamics. We are broadly inter
 ested in answering open questions about function and mechanism in microbio
 logy by measuring structure\, dynamics\, and cooperativity in live bacteri
 al cells via next-generation super-resolution imaging tools. I will discus
 s how we are measuring and understanding the dynamical interactions essent
 ial for DNA mismatch recognition and DNA replication in living cells\, as 
 well as our ongoing work to extend our targets from single cells to microb
 ial communities. Still\, the resolution of single-molecule imaging\, and t
 hus our ability to understand subcellular dynamics\, is limited by the flu
 orescent probes. Thus\, we take advantage of the localized surface plasmon
  resonances that result from the interaction of light with metal nanoparti
 cles to improve the brightness and photostability of nearby fluorescent la
 bels. We have measured the fundamental properties of plasmon-enhanced fluo
 rescence with single-molecule detection\, and we have discovered how coupl
 ing leads to a predictable shift of the emission position. Finally\, we ar
 e applying this understanding to biocompatible enhancement of fluorescent 
 protein emission\, extending the advantages of metal-enhanced fluorescence
  to live-cell bio-imaging\, and creating a flexible technology for high-re
 solution\, real-time imaging.\n
DTSTART:20170425T170000Z
DTEND:20170425T181500Z
LOCATION:Room 1B45\, Burnside Hall\, CA\, QC\, Montreal\, H3A 0B9\, 805 rue
  Sherbrooke Ouest
SUMMARY:Chemical Society Seminar: Dr. Julie Biteen - Single-Molecule Imagin
 g and Plasmon-Enhanced Fluorescence: Understanding Bacterial Function on t
 he Nanoscale
URL:/chemistry/channels/event/chemical-society-seminar
 -dr-julie-biteen-single-molecule-imaging-and-plasmon-enhanced-fluorescence
 -265273
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