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DTSTAMP:20250626T161328Z
DESCRIPTION:How macromolecules behave in nanoconfined spaces\, such as what
  conformations DNA polymers adopt and how they interact with other DNA and
  protein molecules\, is of immense fundamental as well as applied interest
 \, warranting new biophysical explorations. In the context of cellular bio
 physics\, compact coiled DNA molecules are squeezed within a nucleus\, pre
 senting open questions regarding their properties and interactions. In the
  context of genomics\, nanofluidic tools often work by squeezing polymers 
 into long nano pipes\, with the hope of directly unraveling the sequence o
 f our genetic code\, using small numbers of intact DNA segments. One holy 
 grail of modern biotechnology is reading\, assembling and understanding si
 ngle-cell genomes with minimal fragmentation. However\, handling and visua
 lizing long and delicate strands of genomic DNA represent key technologica
 l challenges\, typically breaking DNA into tiny pieces.\n\nIn this talk\, 
 we harness capabilities of a novel single-molecule manipulation and micros
 copy platform we have developed called “Convex Lens-induced Confinement (C
 LiC)” for two explorations in biophysics. First\, we demonstrate a new and
  gentle approach to directly manipulate and visualize long\, purified stra
 nds of DNA for genomic analysis which is high-throughput\, compatible with
  in-situ reagent exchange and controlled chemistry in nanofluidic environm
 ents\, and relies on tiny entropic forces (Berard et al\, PNAS 2014). We p
 recisely explore the polymer physics underlying the DNA polymers’ behaviou
 r as a function of applied confinement\, and compare results to theory\, w
 hich we consequently extend. Second\, using more complex supercoiled DNA s
 ystems\, closer to physiological conditions\, we study how their conformat
 ional fluctuations mediate invasion into spontaneous unwinding sites by sm
 all oligonucleotides. Motivated by open questions on transcriptional initi
 ation and dynamics\, and beginning with model systems\, our single-molecul
 e reaction and trajectory visualizations can provide new mechanistic insig
 hts. The overarching vision of this talk is that by “getting into that roo
 m at the bottom” of nanobiophysics by innovative technologies\, we open do
 ors to complementary biophysical discovery and biomedical diagnostics whic
 h can act hand in hand.\n
DTSTART:20160126T180000Z
DTEND:20160126T193000Z
LOCATION:Rm 10\, Maass Chemistry Building\, CA\, QC\, Montreal\, H3A 0B8\, 
 801 rue Sherbrooke Ouest
SUMMARY:Chemical Society Seminar: Dr. Sabrina Leslie
URL:/chemistry/channels/event/chemical-society-seminar
 -dr-sabrina-leslie-257562
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