BEGIN:VCALENDAR VERSION:2.0 PRODID:-//132.216.98.100//NONSGML kigkonsult.se iCalcreator 2.20.4// BEGIN:VEVENT UID:20250625T093256EDT-07904DuLsl@132.216.98.100 DTSTAMP:20250625T133256Z DESCRIPTION:Abstract:\n\nCutting-edge\, super-resolution fluorescence micro scopy\, combined with the rational design of fluorogenic probes provides a unique opportunity to study chemical processes inside living cellular sys tems\, in a minimally invasive manner with unprecedented spatio-temporal r esolution. To begin\, I give an overview of the work conducted during my d octoral research\, which focused on the development and application of flu orogenic probes and fluorescence microscopy methodologies for rationalizin g the chemistry and biology of lipid oxidation at the cellular level. This work extends the concepts of fluorogenic probe design to developing activ atable photosensitizers\, as a means to exacerbate and ultimately probe th e role of lipid oxidation in biology. The work focused on three major goal s: 1) to improve on current methods of developing fluorogenic probes throu gh a greater understanding of the fundamental photochemistry and photophys ics underlying the sensing mechanisms (Lincoln et al. 2014\, Lincoln et al . 2015)\; 2) to develop novel fluorogenic probes and imaging methodologies for studying the chemistry of lipid oxidation products in cell signaling (Lincoln et al. 2017)\; and 3) to exploit our understanding of the mechani sms of lipid oxidation to develop novel chemically-activated photosensitiz ers as tools to probe lipid oxidation in live cell systems (Lincoln et al. 2017\, Lincoln et al. 2019).\n\nFollowing this\, I will address new oppor tunities in super-resolution microscopy stemming from MINFLUX\, a recently reported methodology by the Stefan Hell lab (Balzarotti et al. 2017). Unl ike stochastic-based super-resolution techniques (e.g. PALM/STORM) which r ely on a large number of emitted photons to calculate the centroid positio n of single fluorescence molecules\, MINFLUX probes the molecular position using a local intensity minimum of excitation light. Thus\, MINFLUX requi res only a modest photon budget to achieve a high localization precision. This enables not only single-nanometer localization precision of fluoresce nt emitters\, but also the rapid tracking of single-fluorescent emitters i n living systems (Eilers et al. 2018). Most recently\, the concept has bee n further extended towards three-dimensional multicolor imaging of living samples (Gwosch et al. 2020). Critical to this technology are the fluoroph ores and labelling strategies employed. In particular\, the desired photoc hemical and photophysical properties: including controllable mechanisms of photoactivation\, consistent photon outputs (i.e. the absence of non-fluo rescent “dark” states)\, and high biocompatibility\, being of particular s ignificance. With the implementation of MINFLUX\, the limit of optical res olution is being fast approached. Now we turn our attention to new challen ges in the field\, notably in the design and synthesis of small and novel fluorogenic probes to aid in achieving this ultimate resolution.\n\nBio:\n \nRichard Lincoln obtained his B.Sc. (Hons.) in Chemistry in 2012 from Aca dia University\, where he worked with Prof. Sherri McFarland studying the photochemistry of ruthenium organometallic complexes. During his undergrad uate\, he travelled to 51Թ on the Reactive Intermediate Stud ent Exchange (RISE) to work with Prof. Gonzalo Cosa studying the photophys ics of BODIPY dyes. After graduating from Acadia\, he returned to the rese arch group of Prof. Cosa to pursue his Ph.D. in chemistry. His research fo cused on developing fluorescence probes to study the chemistry of lipid ox idation. Richard is currently a postdoctoral researcher at the Max Planck Institute for Medical Research in Heidelberg in the research group of Prof . Stefan Hell. He is currently developing novel fluorophores for MINFLUX n anoscopy.\n DTSTART:20200211T180000Z DTEND:20200211T193000Z LOCATION:Room 10\, Maass Chemistry Building\, CA\, QC\, Montreal\, H3A 0B8\ , 801 rue Sherbrooke Ouest SUMMARY:The 2020 Winkler Award Lecture: Richard Lincoln - Lighting and Enli ghtening - the Chemistry of the Cell by Rational Design of Fluorogenic Dye s URL:/chemistry/channels/event/2020-winkler-award-lectu re-richard-lincoln-lighting-and-enlightening-chemistry-cell-rational-desig n-303029 END:VEVENT END:VCALENDAR